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Sampling Procedure

Collecting sample for agricultural analysis is extremely important since the sample must correctly represent the area about which information is desired. Different cultivars (varieties) should be sampled and tested separately. The plantation of same cultivar but with different age also should be sampled separately.

Soil sample

Plow depth or the plow zone is the layer most commonly sampled for soil fertility evaluation. The plow zone is the layer of greatest activity in the soil, and correctly evaluating the properties of this layer go far in predicting fertilizer requirements. Lower horizons in the soil are important too, but they do not change much from one year to another. If basic information is available relative to characteristics of the soil from the plow depths usually will suffice for soil fertility purposes. To obtain a representative sample of a given area of soil, a large number of cores or sub samples must be taken. For most cultivated fields, each composite sample should consist of a minimum of 8-10 cores About 500grams of soil is needed for a routine analysis.

Petiole Sample

A time for sampling must be selected when leaf nutrient levels are relatively stable and best reflects the nutrient needs of the tree. In case of grape, general practice is to collect petiole samples, 45 days after pruning. We, over a period of 17 years evaluated samples at various stages after pruning and data so generated were successfully integrated in computer program.  Samples can be sent at any given point of time stating the date of pruning, our program will take care of the rest. Select fully expanded recently matured, mid cane leaves. Collect 1-2 leaves with petiole from one tree and make about 100 leaves per sample plot. In order to prevent spoilage of the plant material, it is important that the sample be thoroughly air-dried prior to mailing to a laboratory for analysis. Place samples in a clean paper or cloth container

Avoid damaged leaves (insect, disease, mechanical). Abnormal-appearing trees should be avoided. Young or senescent leaves should be avoided. When sampling plants with suspected nutrient deficiencies, take two samples, one from the normal plants and one from the abnormal plants.

Water Sample

Run the water for at least 5 minutes before collecting the sample. Collect 1 litre of sample in a clean bottle.

Complete the Sample submission form as accurately as possible. The more complete the questionnaire is filled out the better the interpretation and recommendation will be.

If you have any questions about sampling procedures, contact:

k_nagda@vsnl.com

 

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