1. Penicillin blocks cell wall synthesis in staphylococcus aureus. A fundamental component utilised in synthesis of the cell wall is (a) N-acetylmuramic acid (b) Muramic acid (c) Hyaluronic acid (d) Mucoitin sulfate (e) Cellulose
2. The tissue with the highest total glycogen content is (a)Muscle (b) Kidney (c) Liver (d) Brain (e) Neerve
3. The major source of ammonia produced by the Kidneys is (a) Leucine (b) Glycine (c) Alanine (d) Glutamine (e) Asparagine
4. Which of the following is NOT a phospholipid ? (a) Cerebroside (b) Pasmogen (c) Sphingomyelin (d) Cephalin (e) Lecithin
5. Chemically a keratin is a (a) Adenine (b) Cystine (c) P-ribose (d) Uracil (e) Phosphate
6. One of the following is NOT an aromatic aminoacid (a) Threonine (b)Phenylalanine (c) Tyrosine (d) Tryptophan
7. Which of the following is a metabolic precursor of uric acid (a) Lysine (b) Hippuric acid (c) Tryptophane (d) Cystine
8. Insulin resistance is encountered in (a) Addison's disease (b) myocardial infarction (c) Hypopituitarism (d) Acromegaly
9. In primaquin sensitivity the enzyme deficient is (a) Aldolase (b) Glucose-6-phosphotase (c) glucose-6-phosphate dehydrogenase (d) Hexokinase
10. High blood cholesterol and diminished sserum proteins are encounterea in (a) Atherosclerosis (b) Hypothyroidism (c) Obstructive jaundice (d) Nephrotic syndrome
11. Convulsive episodes occur when ther is a severe deficiency of (a) Folic acid (b) Pyridoxine (c) Thiamine (d) Riboflavine
12. ''Prothrombin time'' of plasma is prolonged by administration of (a) Vitamin K (b) Dicoumarol (c) Calcium gluconate (d) Folic acid
13. Methyl malonic aciduria is seen in a deficiency of (a) Vitamin B₆ (b) Folic acid (c) Thiamine (d) Vitamin B ₁₂
14. GABA (Gamma amino butyric acid) is (a) Post-synaptic excitatory transmitter (b) Post-synaptic inhibitory transmitter (c) activator of glia-cell function (d) Inhibitor of glia cell function
15. Xanthinoric acid is the metabolite in the metabolism of (a) Xanthine (b) Tryptophan (c) Uric acid (d) Uronic acid
16. The important metabolic link between the major pathways of carbohydrate metabolism is (a) Acetyl-CoA (b) Lactate (c) Oxaloacetate (d) Pyruvate
17. The richest known source of prostaglandins include (a) Blood (b) Seminal fluid (c) Menstrual fluid (d) Cerebrospinal fluid
18. Which of the following vitamin is present in Co-enzyme A ? (a) Thiamine (b) Pyridoxine (c) Nicotinic acid (d) Pantothenic acid
19. Sulpha drugs are detoxified by conjugation with (a) Acetic acid (b) Glucoronic acid (c) Methionine (d) Cysteine
20. The Binding protein of Retinol- is (a) Albumin (b) Alpha albumin (c) Beta1 globulin (d) Gamma globulin
21. Which of the following accumulates in various tissues in Gaucher's disease ? (a) Glycogen (b) Glucocerebroside (c) Sphingomyelin (d) Galactose
22. Formiminoglutamic acid (FIGLU) formed in the metabolism of (a) Glysine (b) Tvrosine (c) Histidine (d) Leucine
23. The aminoacid which causes hypoglycemia is (a) Valine (b) Lysine (c) Leucine (d) Phenylalanine
24. The rate limiting step in 'glycolysis' is controlled by (A)Aldolase (B)Pyruvate kinase (C)Phosphofructokinase (D) Glycerophosphokinase
25. Which of the following is not an essential amino acid (A) Leucine (B)Isoleucine (C)Tyrosine (D) Phenylalanine
26. Rate limiting step in 'cholesterol biosymtheis' is mediated by (A)HMG CoA synthetase (B)HMG CoA reductase (C)Mevalonate kinase (D) Squalene synthetase
27. Gibbs Donnan Equilibrium determines (A) Distribution of solutes separated by a permeable membrane (B)Distribution of solvents separated by a permeable membrane (C)Distribution of both solvents and solutes separated by a semipermeable membrane (D) Effect of non ionic solutes on distribution of ionic solutes separated by a semipermeable membrane
28. Coenzyme A is derived from (A)Pantothenic acid (B)Riboflavin (C)Niacin (D) Pyridoxine
29. Glycine is precursor of (A)Purine (B)Creatine (C)Heme (D) All of the above
30. Components of 'pyruvate dehydrogenase comples' are all except (A) Pyruvate decarboxylase (B)Pyruvate kinase (C)Dihydrolipoyl transcetylase (D) Dihydrolipoyl dehydrogenase
31. Glyconeogenesis occurs in (A)Liver (B) Kidney (C)Small intestine (D) All of the above
32. Which of the following occurs in mitochondria (A) Cholesterol biosynthesis (B)Ketone body formation (C)Fatty acid synthesis (D) Pentose phosphate pathway
33. Rate of muscle protein loss can be measured by urinary (A)3-methyl histidine (B)Urea (C)Uric acid (D) Creatine
34. Which of the following is not required for purine biosynthesis (A) Glycine (B)Aspartate (C)Glutamine (D) Gumarate
35. Metabolic effects of thyroid hormones are seen on all tissues except (A)Liver (B)Kidney (C) Testis (D) Adipose tissue.
36. Replacement of a purine by a pyrimidine or vice versa is (A)Transition (B)Transversion (C)Deletion (D) Insertion
37. Enzyme that catalyzes the conversion f an aldose sugar to a ketose sugar is (A)Transferase (B)Hydrolase (C)Oxidoreductase (D) Isomerase
38. All are fat soluble vitamins except (A)C (B) D (C) E (D) K
39. A change of pH by 1 unit causes a change in alkalinity or acidity by (A) 2 times (B)3 times (C)10 times (D) 100 times
40. All are disaccharides, except (A) Lactose (B)Maltose (C)Mannose (D) Sucrose
41. Exact amino acid sequence in a protein molecule can be determined by (A)Ninhydrin reaction (B)Sanger's method (C)Edman's method (D)Formol titration method.
42. 'Allosteric inhibition' is seen with (A) Competitive inhibition (B)Non-competitive inhibition (C)Uncompetitive inhibition (D)Feed back inhibition.
43. Iron absorption is decreased by all except (A) Phosphates (B)Oxalates (C) Phytates (D) Ascorbic acid
44. Best biochemical test for vitamin B1 deficiency is (A)Rise in serum pH (B) Rise in serum pyruvic acid (C) Rise in serum glucose (D) Rise in serum bicarbonate
45. Benedict's solution is not reduced by (A) Lactose (B)Maltose (C)Fructose (D) Sucrose
46. Actionomycin is inhibitor of (A)Transduction (B)Transcription (C)Transcription (D) All of the above.
47. Metabolic precursor of uric acid is (A) Xanthureic acid (B)Hippuric acid (C) Homogenestic acid (D) Acetoacetate
48. In metabolic acidosis, primary abnormality is (A) Sodium ion deficiency (B)Bicarbonate deficiency (C)Bicarbonate excess (D)CO2 excess.
49. During prolonged exercise, caloric needs of skeletal muscle are met by (A)Increased hepatic glycogenolysis (B)Increased muscle gluconeogenesis (C) Increased intestinal glucose absorption (D) Release of free fatty acids from adipose tissue.
50. Most active form of vitamin D is (A)24, 25 dihydroxycholecalciferol (B)1, 25 dihydroxycholecalciferol (C)25 hydroxycholecalciferol (D) 25 hydroxyergocaliferol.
51. Tissue which is completely dependent upon glucose as fuel source is (A) Retina (B)Heart (C)Renal cortex (D) Adipose tissue
52. Which of the following combines with opsin to form rhodopsin (A) Cis-retinol (B)Cis-retinal (C) Trans-retinal (D)Trans-retinaldehyde
53. Green precipitate in Benedict's qualitative test indicates that the amount of reducing sugar present is (A) <0.5 g%(B)0.5-1g% (C)1.5-2.0g% (D)>2.0g%
54. Muscle can utilize ketone bodies as a source of energy because they can convert (A) Acetoacetate to acetoacetyl CoA (B) Acetoacetate to betahydroxyputyrate (C)Acetone to dihydroxy acetone (D) Acetoacetate to acetone.
55. Normal plasma level of ketone bodies in (A) 0.3-2.0 mg/100ml (B)2.0-3.0 mg/100 ml (C)5.0-10 mg100ml (D) 10-20 mg/100 ml.
56. Irreverssible step in glycolytic pathway is mediated by all except (A) Aldolase (B)Hexokinase (C)Pyruvate kinase (D)phosphofructokinase.
57. Which of the following statement about 'denaturation' of proteins is not true (A)There is dissocation of subunit peptide chain (B)There is unfolding of chains into random coils (C)The primary sturcture is also disturbed (D) There is partial or complete loss of biological activity of protein.
58. The process of separation of crystalloids from colloids by diffusion through a membrane is called (A) Precipitation (B)Dialysis (C)Tyndallisation (D) Brownian effect.
59. Radioavtive labelling of DNA can be achieved by using tritiated (A)Adenine (B)Thymine (C)Uracil (D) Guanine.
60. Which of the following statement is not true about enzymes (A) Consists of subunits (B)Destroyed by heat (C)Has primary structure (D) Has a 3 dimensional structure.
61. Which of the following statement about 'isoenzymes' is not true (A)They have different molecular weights (B)Catalyse the same reaction but differ in Km (C)Depressed in same manner by inhibitors (D) CPK (BB) is predominantly found in brain tissue.
62. Fructose in the seminal fluid is synthesized in (A)In hibiting glycogenesis (B) Decreasing oxidation of glucose (C)Increased absorption of glucose from intestine (D) Increased glycogenolysis in liver.
63. Amount of cholesterol synthesized per day is (A) 0.3-lg (B) 1-1.5g (C)2-4g (D)10-15g.
64. Insulin stimulates the synthesis of which of the following enzyme (A) Pyruvate (B)Glucose-1-phosphatase (C)Pyruvate carboxylase (D) Phosphory lase.

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